Manufacture and production of a nutrient medium for use in fermentation processes.



' U ITED sTATEs PATENT OFFICE.

AUous'rE FERNBACH, or rams, FRANCE, AND EDWARD HALFORD s'rEANGE, or .LbNDoN, ENGLAND.

MANUFACTURE AND PRODUCTION OF NUTRIENT MEDIUM FOR USE IN I FEBMENTATION PROCESSES.

Specification of Letters Patent.

Patented Nov. 12, 1,912.

No Drawing. Original application filed September 24, 1912, Sefial No. 722,120. Divided and this application filed October 10, 1912. Serial No. 724,989.

.To all whom it may concern:

Be it known that we, Aueus rn FERNBACII,

acitizen of the Republic of France, of 26 Rue Dutot, Paris, in the Republic of France,

' Use in Fermentation Processes, of which the following is a specification.

The present application is a division of our co-pending application, Serial No. 722,120, filed September 24,1912, for. fermentation process for the production of v acetone.

i The object of our invention 1s to manufacture a nutrient medium for use in fermentation processes, the-said medium enabhng'fermentatlon to be carried on in a ,very economical and 'eflicient manner, and

with a good yield. The said medium is made from yeast, (such for instance-as yeast remaining from processes of fermentation) by mixing it with water and sterilizing it, which can be done by subjectingit to a sutficiently high temperature for some time,

and then the mixture is cooled and a ferment of the type of Tyrothriw tennis is added, sterilized air being allowed to have access to the mixture during, or at intervals during, the eriod of fermentation. At the end of the ermentation it has been fpund that about ninety per cent. of the nitrogenous. matter of the yeast has been converted into a soluble form not precipitated by tannin.

The following is an example ofthe way in which our invention can-be performed, but

we do not limit ourselves to this example:

100 pounds of pressed yeast are mixed with 1,000 pounds of water, and the mixture is sterilized by heat, employing a temperature of, say, about 130 centigrade during several hours. The mixture is then cooled, preferably to a temperature of from 30 to 35 centigrade and a ferment of the'type Tyrot/wiw team's is added, in the presence of sterilizedair, care being taken that no other ferment obtains access to the mixture. The mixture is -maintained preferably at from 30 to 35 Centigrade for from four, to five, weeks, sterilized air being admitted, say at intervals of 24 hours. By the end of the four or five weeks, about ninety per cent. of the nitrogenous content of the mixture is converted into compounds which are not capable of being precipitated big tannin.

What we claim is:

-1. The process of preparing a nutrient medium for use in a fermentation process which consists in mixing yeast with water and subjecting the mixture to the action of a ferment of the type of Tg mthriaa tennis in the presence of sterilized air.

2. Theprocess of preparing. a nutrient medium for use in a fermentation process Which consists in mixing yeast with water, sterilizing the mixture, and subjecting the mixture to the action of a ferment of the type of Tyrothm'w team's in the presence of sterilized air. I a

3. A nutrient medium for fermentation processes, comprising yeast having substantially-- 90% of its nitrogenous matter con verted into a soluble form not precipitable bytannin, r

- In testimony whereof we have signed our names tothis. specification inthe presence of two subscribing witnesses.

AUGUSTE FERNBAOH. EDWARD HALFORD sTRANeE.

Witnesses: LUomN MEMMINGER, JACK G. BAKER. 

